Open AccessPassive transfer of protection against cholera toxin in rat intestine
Author(s) -
Lange Stefan,
Lönnroth Ivar
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01298.x
Subject(s) - cholera toxin , cholera , toxin , microbiology and biotechnology , chemistry , virology , biology
Passive transfer between rates of protection against cholera toxin (CT) was studied. Extracts of various organs, obtained from CT‐immunized rats, were injected intravenously into non‐immunized recipient rats. The ability of the extracts to inhibit CT‐induced secretion in ligated jejunal loop were tested. A significant inhibition of the response to CT was achieved by extracts from hypophysis, brain and jejunal mucosa. Extracts from pancreas, spleen or adrenal glands were without effect, as were all extracts obtained from control rats. The antisecretory effects of the hypophysis extracts became intensified with increasing numbers of immunizations, and the antisecretory effect was most pronounced when the extract was injected immediately before the CT challenge. The active component of the hypophysis extract was heat‐labile and negatively charged, suggesting an acidic protein as the mediator of the protective effect against CT.