Open AccessDielectrophoretic behaviour of Saccharomyces cerevisiae : effect of cations and detergents
Author(s) -
López Ma Carmen,
Iglesias Francisco J.,
Santamaria Carlos,
Domínguez Angel
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01295.x
Subject(s) - saccharomyces cerevisiae , cetrimide , yield (engineering) , yeast , electrokinetic phenomena , conductivity , chemistry , electrophoresis , electrical resistivity and conductivity , analytical chemistry (journal) , materials science , chemical engineering , nanotechnology , chromatography , biochemistry , physics , composite material , medicine , dentistry , chlorhexidine , quantum mechanics , engineering
The dielectrophoretic yield of cell suspensions of Saccharomyces cerevisiae shows a maximum for a given frequency and this maximum shifts towards higher frequencies as the electrical conductivity of the medium increases. The addition of cations to cell suspensions of S. cerevisiae , maintaining the electric conductivity constant, leads to a decrease in the dielectrophoretic yield, depending of the valence of the cations added. Both results could be interpreted by a modified Maxwell‐Wagner mechanism. Cetrimide is the most efficient detergent in permeabilizing yeast cells and the dielectrophoretic yield could be used as a method for quantifying cellular viability.