Open AccessGrowth characteristics of Pseudomonas strains carrying catabolic plasmids and their cured derivatives
Author(s) -
Clarke Patricia H.,
Laverack Peter D.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01254.x
Subject(s) - indigo , plasmid , indole test , pseudomonas , bacteria , benzoic acid , chemistry , pseudomonadales , microbiology and biotechnology , pseudomonadaceae , biology , stereochemistry , biochemistry , gene , genetics , art , visual arts
Cured derivatives of Pseudomonas strains carrying TOL or NAH plasmids were obtained by selection on plates containing benzoic acid at almost the inhibitory concentration. Bacteria containing TOL and NAH plasmids produced deep blue colonies on m ‐toluate/indole or naphthalene/indole plates and weaker reactions on benzoate/indole plates. The indigo reaction of the TOL strains was ascribed to the plasmid‐coded benzoate/toluate oxidase. Strains that had been cured of their plasmids did not produce indigo.