Open AccessPrime plasmids derived from the IncP‐10 plasmid R91‐5 in Pseudomonas putida
Author(s) -
Cain D.,
Holloway B.W.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01252.x
Subject(s) - plasmid , biology , pseudomonas putida , transformation (genetics) , chromosome , genetics , plasmid preparation , microbiology and biotechnology , dna , bacteria , gene , pbr322
Plasmid primes carrying various fragments of Pseudomonas putida chromosome have been derived from pMO22, a derivative of R91‐5 loaded with Tn 501 . These prime plasmids transfer efficiently to P. aeruginosa where they effectively complement various auxotrophic markers. Proof of prime plasmid formation has been provided by the high‐frequency transfer of plasmid and chromosomal markers, the unselected cotransfer of either plasmid or chromosomal markers into P. aeruginosa and by transformation of both plasmid and chromosomal markers using prime plasmid DNA. Such prime plasmids have been used to map the location of new markers on the P. putida chromosome.