Open AccessMolecular cloning of the Escherichia coli K‐12 entACGBE genes
Author(s) -
Pickett Carol L.,
Hayes LaDonna,
Earhart Charles F.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01248.x
Subject(s) - escherichia coli , complementation , plasmid , operon , restriction map , gene , biology , restriction enzyme , dna , cloning (programming) , molecular cloning , genetics , microbiology and biotechnology , restriction site , ecori , gene expression , computer science , programming language , phenotype
A 7‐kb piece of Escherichia coli DNA that contains five genes ( entA, C, G, B and E ) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini‐ and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an M r 63000 polypeptide.