Open AccessProduction of the ferripyochelin outer membrane receptor by Pseudomonas species
Author(s) -
Sokol Pamela A.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01085.x
Subject(s) - pseudomonas stutzeri , pseudomonas putida , siderophore , pseudomonadaceae , bacteria , pseudomonas , chemistry , pseudomonadales , membrane , bacterial outer membrane , thin layer chromatography , biology , microbiology and biotechnology , chromatography , biochemistry , escherichia coli , genetics , gene
Abstract Production of pyochelin by Pseudomonas species was measured in ethyl acetate extracts of culture supernatants. Pyochelin was purified by paper chromatography, followed by two‐dimensional, thin‐layer chromatography. Strains of P. cepacia , P. multivorans , and P. fluorescens produced pyochelin, while P. stutzeri , P. putida and P. maltophila were negative for pyochelin production. Outer membranes prepared from these strains had a protein of M r 14000 which was shown to bind [ 59 Fe]pyochelin.