Open AccessAssay for acetyl coenzyme A: Alkylhydroxylamine N ‐acetyltransferase in Klebsiella pneumoniae ATCC25304
Author(s) -
Kusters J.,
Diekmann H.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01084.x
Subject(s) - lysine , acetylation , acetyltransferase , acetyl coa , hydroxylysine , klebsiella pneumoniae , biochemistry , ornithine , coenzyme a , chemistry , stereochemistry , enzyme , amino acid , arginine , escherichia coli , reductase , gene
An alkylhydroxylamine N ‐acetyltransferase was partially purified from extracts of Kelbsiella pneumoniae . N 6 ‐Hydroxylysine, N 5 ‐hydroxyornithine and N ‐methylhydroxylamine were acetylated with decreasing velocity. N 6 ‐Acetyl‐ l ‐lysine showed noncompetitive inhibition while l ‐ornithine, l ‐lysine, their α‐acetyl derivatives and N 5 ‐hydroxylysine were without effect at 0.1 M. A sensitive assay using 14 C‐labelled acetyl coenzyme A was developed.