Open AccessA mutant of Halobacterium halobium with constitutive production of bacteriorhodopsin
Author(s) -
Juez Guadalupe,
RodriguezValera F.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01055.x
Subject(s) - bacteriorhodopsin , mutant , strain (injury) , microaerophile , halobacterium salinarum , wild type , chemistry , biophysics , biology , biochemistry , bacteria , anatomy , genetics , membrane , gene
A purple mutant of Halobacterium halobium was isolated in a previous study. The ‘in vitro’ absorption spectra of the cells gave a broad shoulder around 570 nm. The amounts of bacteriorhodopsin were high under any growth condition (including aerobic) inhibitory for the wild‐type strain. The mutant grew faster under illuminated microaerophilic conditions and showed faster proton extrusion than the wild‐type strain. This evidence shows that the mutant has a constitutive bacteriorhodopsin production not influenced by the oxygen concentration in the medium. However, some stimulation by light was found.