Open AccessAnalysis of penicillin‐binding sites with a micro method — The binding site of PBP 5 from Escherichia coli
Author(s) -
Glauner Bernd,
Keck Wolfgang,
Schwarz Uli
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01052.x
Subject(s) - penicillin binding proteins , escherichia coli , penicillin , serine , chemistry , binding site , biochemistry , chromatography , enterobacteriaceae , microbiology and biotechnology , biology , antibiotics , enzyme , gene
A micro method for the isolation and characterization of the penicillin‐binding sites in penicillin‐binding proteins (PBPs) was developed. Only 10 nmol of a pure PBP are required for the whole procedure which is based on high‐pressure liquid chromatography (HPLC). We showed that serine 44 in PBP 5 from Escherichia coli binds penicillin covalently.