Open AccessRegulation of nitrogen fixation (nif) genes of Azospirillum brasilense by nifA and ntr (gln) type gene products
Author(s) -
Pedrosa F.O.,
Yates M.G.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01042.x
Subject(s) - nitrogenase , azospirillum brasilense , mutant , nitrogen fixation , mutagenesis , structural gene , klebsiella pneumoniae , gene , biochemistry , biology , wild type , microbiology and biotechnology , bacteria , chemistry , escherichia coli , genetics
Eight Nif − mutants of Azospirillum brasilense were obtained by N ‐nitrosoguanidine mutagenesis and isolated by growth on glutamate medium. Three of these mutants had no nitrogenase activity, possessed no nitrogenase structural proteins and were complemented by Klebsiella pneumoniae nifA . Evidence will be presented that one of these mutants is defective in a nifA type regulatory gene but the other two were also complemented by K. pneumoniae ntrC and may be ntrC − ‐type mutants. A fourth mutant was defective in the MoFe component protein of nitrogenase.