Open AccessComplementation of succinate dehydrogenase mutants in fused Bacillus subtilis protoplasts
Author(s) -
Hederstedt Lars,
Franzén SvenÅke
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01034.x
Subject(s) - complementation , bacillus subtilis , mutant , succinate dehydrogenase , biochemistry , protoplast , cytoplasm , biology , chemistry , enzyme , gene , genetics , bacteria
Succinate dehydrogenase (SDH) and cytochrome b 558 are present in Bacillus subtilis cytoplasmic membranes as a complex consisting of three different subunits. Complementation of membrane‐bound SDH activity was studied in fused protoplasts of two SDH‐negative mutants mutated in different subunits of the complex. The complementation was found to be rapid, temperature dependent and not sensitive to inhibitors of respiration. It is concluded that the complementation predominantly results from the assembly of preformed SDH subunits.