Open AccessDNA‐binding proteins specified by bacteriophage T1
Author(s) -
Ritchie D.A.,
Joicey D.H.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01024.x
Subject(s) - dna , biology , dna binding protein , gene , microbiology and biotechnology , dna clamp , gel electrophoresis , bacteriophage , in vitro recombination , dna replication , single stranded binding protein , replication protein a , biochemistry , molecular cloning , polymerase chain reaction , escherichia coli , gene expression , transcription factor , reverse transcriptase
DNA affinity chromatography with native DNA‐cellulose and denatured DNA‐Sepharose followed by SDS‐polyacrylamide gel electrophoresis was used to identify phage T1‐coded proteins which bind to DNA. Nine proteins were found to bind strongly to calf thymus DNA, all of which belonged to the Class I (early) or Class II (early‐late) categories of T1‐specified gene products. The products of T1 genes 1 and 2, which are essential for T1 DNA synthesis, and of genes 3.5 and 4 which function in DNA replication and recombination, all bound strongly to DNA. None of the T1 structural proteins were identified as having DNA‐binding affinity.