Open AccessThe nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli
Author(s) -
Roosendaal Bert,
Gaastra Wim,
Graaf Frits K.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00737.x
Subject(s) - enterotoxigenic escherichia coli , protein subunit , escherichia coli , nucleic acid sequence , peptide sequence , biology , gene , amino acid , nucleotide , sequence (biology) , microbiology and biotechnology , biochemistry , genetics , enterotoxin
The nucleotide sequence of the gene encoding the K99 fimbrial subunit of enterotoxigenic Escherichia coli was determined. It appeared that the subunit is composed of 159 amino acid residues preceded by a N‐terminal signal sequence of 22 amino acid residues. The secondary structure of the mature K99 polypeptide and the location of potential antigenic determinants were predicted. A comparison was made between the amino acid sequence of the K99 subunit and the subunits of other fimbrial adhesins.