Open AccessAnalysis of electrophoretically heterogeneous lipopolysaccharides of Escherichia coli by immunoblotting
Author(s) -
Karch Helge,
Leying Hermann,
Opferkuch Wolfgang
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00724.x
Subject(s) - escherichia coli , antiserum , heterologous , staining , electrophoresis , silver stain , polyacrylamide gel electrophoresis , microbiology and biotechnology , gel electrophoresis , biology , lipopolysaccharide , enterobacteriaceae , bacteria , chemistry , antigen , biochemistry , genetics , gene , enzyme , immunology
The purified lipopolysaccharide (LPS) from each of four Escherichia coli serogroups which was separated by sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and visualized by periodic acid‐silver staining, exhibited a different distribution of bands. The same serogroup‐specific banding patterns were evident on immunoblots developed with homologous but not heterologous O‐antisera. Thus, the electrophoretic heterogeneity of LPS was confirmed and the multiple bands seen on SDS‐PAGE further shown to contain O‐serogroup‐specific antigenic determinants.