Open AccessIntroduction of the mercury transposon Tn501 into Rhizobium japonicum strains 31 and 110
Author(s) -
Bullerjahn G.S.,
Benzinger Rolf H.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00722.x
Subject(s) - transposable element , transposon mutagenesis , genetics , biology , transposition (logic) , plasmid , dna transposable elements , gene , mutant , linguistics , philosophy
Transposon Tn 501 , which encodes resistance to mercuric ions, was introduced into Rhizobium japonicum 110 and 31 by conjugal transfer. The transposon donor plasmid (pMD100) was able to mobilize into R. japonicum , but could not be maintained. Hg 2+ ‐resistant colonies were recovered at a frequency of 1.9 × 10 −8 /recipient for strain 110, and 1.7 × 10 −7 /recipient for strain 31. Presence of Tn 501 in Hg‐resistant isolates was verified by Southern analysis and demonstrating transposition of Hg resistance. Transposon mutagenesis has been used to generate auxotrophic mutations at low frequency.