Open AccessThe effect of antibiotic treatment on the in vivo selection of resistant haemolytic Escherichia coli clones in mice
Author(s) -
Emody Levente,
Kerényi Monika,
Bátai István
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00721.x
Subject(s) - escherichia coli , microbiology and biotechnology , carbenicillin , antibiotics , kanamycin , biology , in vivo , plasmid , enterobacteriaceae , ampicillin , biochemistry , gene
The appearance of resistant haemolytic Escherichia coli clones was studied in mice treated with a synergistic combination of antibiotics. The R plasmid donor and the haemolytic recipient were given orally. Ten animals were treated i.p. with 2.5 mg carbenicillin and 2.5 mg kanamycin pro die for 7 days. Ten control mice were injected with saline. Out of 80 faecal samples of animals receiving antibiotic treatment resistant haemolytic transconjugants were isolated from 13 samples by direct plating, and from 27 samples after selective enrichment. Out of 80 parallel samples of control animals a single one yielded a positive culture — after enrichment.