Open AccessThe metC gene in Escherichia coli K‐12: Isolation and studies of relatedness in Enterobacteriaceae
Author(s) -
Michaeli Shulamit,
Ron Eliora Z.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00349.x
Subject(s) - ecori , plasmid , pbr322 , hindiii , enterobacteriaceae , escherichia coli , biology , genetics , homology (biology) , gene , restriction enzyme , bamhi , microbiology and biotechnology
A specialized transducing phage λ carrying the structural gene for β‐cystathionase ( metC ) of Escherichia coli was isolated. The phage carries a 21‐kb fragment of the E. coli K‐12 chromosome, and its structure was analyzed using restriction enzymes. The metC gene was recloned into resistance plasmid pBR322, using Eco RI or Hin dIII. The information for the metC gene is contained in a 1.3‐kb fragment, which shows a high degree of homology with representative strains of all tribes of Enterobacteriaceae.