Open AccessEvidence for the presence of cyclic adenosine monophosphate in Bacillus subtilis
Author(s) -
Mach Hiltraut,
Hecker Michael,
Mach Friedrich
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00348.x
Subject(s) - bacillus subtilis , phosphodiesterase , adenylate kinase , adenosine , cyclase , biochemistry , chemistry , cyclic adenosine monophosphate , in vivo , biology , enzyme , bacteria , receptor , genetics , microbiology and biotechnology
Cells of B. subtilis are able to synthesize cAMP in response to oxygen limitation. Several methods were used to characterize cAMP in cell extracts. Using [ 3 H]adenosine, cAMP synthesis could also be detected under in vivo conditions. Furthermore, activities of phosphodiesterase and adenylate cyclase were measured in extracts of B. subtilis cells and in membrane fractions. We suggest that in B. subtilis cAMP is synthesized only under special physiological conditions.