Open AccessNovel antibiotic hypersensitive mutants of Escherichia coli genetic mapping and chemical characterization
Author(s) -
Clark David
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00209.x
Subject(s) - escherichia coli , cell envelope , mutant , lipopolysaccharide , chloramphenicol , biochemistry , chemistry , antibiotics , microbiology and biotechnology , mutation , biology , gene , endocrinology
The abs mutants of Escherichia coli showed increased sensitivity to chloramphenicol, β‐lactams, and many other unrelated antibacterial agents. The cell envelope was demonstrated to be more permeable to several β‐lactams and dyes. The antibiotic hypersensitivity could be phenotypically suppressed by fucose and other deoxyhexoses. The abs mutation was located between Mel and purA at 94 minutes on the E. coli genetic map. The content of ethanolamine in the lipopolysaccharide increased in the abs mutant but the sugar fatty acid and phosphate composition of the lipopolysaccharide were unaltered. In addition, minor quantitative changes in envelope protein composition were observed.