Open AccessPlasmid‐coded degradation of ethylbenzene and 1‐phenylethanol in Pseudomonas fluorescens
Author(s) -
Bestetti G.,
Galli E.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00204.x
Subject(s) - ethylbenzene , pseudomonas fluorescens , plasmid , mutant , agarose gel electrophoresis , pseudomonadales , agarose , chemistry , pseudomonadaceae , biochemistry , microbiology and biotechnology , pseudomonas , biology , gene , bacteria , genetics , catalysis
Pseudomonas fluorescens EB carries genes for the catabolism of ethylbenzene and 1‐phenylethanol on a plasmid. The size of the plasmid as measured by analysis of agarose electrophoresis gels after restriction endonuclease hydrolysis, was 253–267 kb. By treatment with Mitomycin C, mutants of EB strain were obtained bearing a plasmid which had undergone an extensive deletion of about 80 kb. These mutants have lost the ability to grow on ethylbenzene and 1‐phenylethanol as well as to synthesize meta‐cleavage enzymes.