Open AccessEvidence that mucoid Pseudomonas aeruginosa in the cystic fibrosis lung grows under iron‐restricted conditions
Author(s) -
Brown Michael R.W.,
Anwar Hosmin,
Lambert Peter A.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00195.x
Subject(s) - cystic fibrosis , pseudomonas aeruginosa , sputum , subculture (biology) , microbiology and biotechnology , polyacrylamide gel electrophoresis , gel electrophoresis , pseudomonadaceae , biology , chemistry , bacteria , pathology , biochemistry , medicine , tuberculosis , genetics , enzyme
The outer membrane protein composition of mucoid Pseudomonas aeruginosa recovered without subculture from the sputum of a cystic fibrosis patient was studied by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. The results indicated that three outer membrane proteins in the range of M r 80 000–90 000 were induced. The induction of these proteins can be simulated by growing the same isolate under iron‐restricted conditions in laboratory media. This initial study gives the first direct biochemical evidence that mucoid P. aeruginosa grows under iron restricted conditions in the lungs of the cystic fibrosis patient.