Open AccessHydrogenosomes in known species of rumen entodiniomorphid protozoa
Author(s) -
Yarlett Nigel,
Coleman Geoffrey S.,
Williams Alan G.,
Lloyd David
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00178.x
Subject(s) - rumen , protozoa , hydrogenase , biochemistry , organelle , biology , enzyme , in vitro , microbiology and biotechnology , fermentation
The distribution of hydrogenosomal enzymes in several species of rumen entodiniomorphid protozoa grown in vivo and in vitro was investigated. Eudiplodinium maggii and Epidinium ecaudatum caudatum were shown to possess pyruvate synthase and hydrogenase enzyme activities associated with a fraction sedimentable at 10 5 / g/min and enriched in granular microbody‐like organelles about 0.3 μm in diameter; these organelles are therefore morphologically and enzymically similar to hydrogenosomes from the rumen holotrichs. However, hydrogenase and pyruvate synthase were undetectable in several of the entodiniomorph species investigated.