Open AccessA novel shuttle cloning vector for the cyanobacterium Anacystis nidulans
Author(s) -
Gendel Steven,
Straus Neil,
Pulleyblank David,
Williams John
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00559.x
Subject(s) - shuttle vector , multiple cloning site , cloning (programming) , plasmid , cloning vector , escherichia coli , biology , molecular cloning , vector (molecular biology) , restriction enzyme , recombinant dna , genetics , microbiology and biotechnology , dna , gene , peptide sequence , computer science , programming language
Shuttle cloning vectors for use with the cyanobacterium Anacystis nidulans and Escherichia coli were constructed by combining an endogenous A. nidulans plasmid with an E. coli vector containing a 14 site non‐symmetrical polylinker. The resulting plasmids, designated pPLAN B1 and pPLAN B2, transform A. nidulans with high efficiency and contain 7 unique restriction enzyme sites suitable for cloning.