Open AccessCotransduction of his and trp loci by phage SV1 in Streptomyces venezuelae
Author(s) -
Stuttard C.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00169.x
Subject(s) - streptomyces coelicolor , genetics , biology , gene , mutagenesis , mutant , genetic linkage , gene cluster , transduction (biophysics) , dehydratase , biochemistry
A new tryptophan auxotroph of Streptomyces venezuelae was isolated by transduction of a histidine mutant to His + after hydroxylamine mutagenesis of the transducing lysate. Cotransduction of at least two his genes and two trp genes was then demonstrated. One trp and two his markers were not cotransducible with this gene cluster, but the separate trp marker, trp ‐4, was cotransducible with a nic marker. These results are compatible with the distribution of similar markers on the conjugational linkage map of S. coelicolor A3(2), and so support the hypothesis of conservation of linkage relationships among putative homologous loci within the genus Streptomyces .