Open AccessSeparation and analysis of free ceramides containing 2‐hydroxy fatty acids in Sphingobacterium species
Author(s) -
Yano Ikuya,
Imaizumi Sadao,
Tomiyasu Ikuko,
Yabuuchi Eiko
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00166.x
Subject(s) - chloroform , chemistry , carbon chain , fatty acid , methanol , carbon fibers , stereochemistry , chromatography , biochemistry , organic chemistry , materials science , composite number , composite material
The occurrence of free ceramides was shown in the chloroform‐methanol extractable lipids of 16 strains of Sphingobacterium including three species: S. versatilis, S. multivorum and S. mizutae . The predominant long‐chain base was identified as a branched‐chain, saturated dihydroxy base with a carbon chain consisting of 17 carbon atoms, while the most abundant fatty acid was 2‐hydroxy‐13‐methyltetradecanoic acid. The major molecular species of the intact ceramides were identified as LCB‐ d ‐ iso ‐17 : 0‐2‐OH iso ‐15 : 0FA, LCB‐ d ‐ iso ‐17 : 0‐ iso ‐15 : 0FA and LCB‐ d ‐n16 : 0‐ iso ‐15 : 0FA.