Open AccessRegulated OmpF porin synthesis in Escherichia coli K‐12
Author(s) -
Pugsley Anthony P.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00165.x
Subject(s) - porin , bacterial outer membrane , escherichia coli , regulon , biology , transcription (linguistics) , lac operon , enterobacteriaceae , microbiology and biotechnology , gene expression , gene , biochemistry , linguistics , philosophy
The effects of growth conditions on the expression of the Escherichia coli K‐12 major outer membrane porin gene ompF were determined by measuring the amount of OmpF protein incorporated into the outer membrane, β‐galactosidase activity in cells in which lacZ was regulated by the ompF promoter, and by studying their effects in the presence of the general transcription inhibitor rifampicin. A variety of conditions reduced ompF transcription. Common features among the inhibitors of ompF expression could not be identified; another protease inhibitor and another uncoupler did not specifically affect ompF expression. It is therefore concluded that the inhibitors interact directly or indirectly with a sensory system (possibly EnvZ protein) which affects the porin gene regulon accordingly.