Open AccessPurification of membrane‐bound polyvinyl alcohol oxidase in Pseudomonas sp. VM15C
Author(s) -
Shimao M.,
Tsuda T.,
Takahashi M.,
Kato N.,
Sakazawa C.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00162.x
Subject(s) - polyvinyl alcohol , pseudomonas putida , chemistry , membrane , phenazine , pseudomonas , chromatography , alcohol , enzyme , oxidase test , alcohol dehydrogenase , biochemistry , organic chemistry , bacteria , biology , genetics
Polyvinyl alcohol (PVA) was utilized by a symbiotic mixed culture which was composed of Pseudomonas putida VM15A and Pseudomonas sp. VM14C. The PVA oxidase was found in the culture fluid, membrane, and cytosol fractions of VM15C. The membrane‐bound PVA oxidase was purified by several steps of chromatography. The enzyme (p I = 9.6) exhibited the maximum activity at pH 8.0 to 8.4 and 45°C, and utilized secondary alcohol as well as PVA. The enzyme showed the PVA dehydrogenating activity linking with phenazine ethosulfate, indicating the possibility that PVA oxidation is coupled with an electron transport chain on the bacterial membrane.