Open AccessPeroxide inducible phage reactivation in Bacteroides fragilis
Author(s) -
Slade Heide J.K.,
Schumann J.Petra,
Jones David T.,
Woods David R.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00156.x
Subject(s) - bacteroides fragilis , microbiology and biotechnology , dna , bacteroidaceae , biology , bacteriophage , irradiation , bacteroides , chemistry , bacteria , escherichia coli , biochemistry , antibiotics , gene , genetics , physics , nuclear physics
Reactivation of UV‐irradiated phage b‐1 was induced by H 2 O 2 and UV in Bacteroides fragilis . The characteristics of H 2 O 2 and UV induced phage reactivation differ from a previously reported oxygen induced reactivation system. The survival of B. fragilis cells after UV irradiation was also increased by pretreatment with H 2 O 2 . DNA synthesis was not inhibited in the host cells exposed to H 2 O 2 concentrations which induced phage reactivation. The pattern of DNA degradation and synthesis after UV irradiation with and without H 2 O 2 differed from the effect of O 2 on DNA synthesis in irradiated B. fragilis cells.