Open AccessCharacterization of exoglucanase and synergistic hydrolysis of cellulose in Clostridium stercorarium
Author(s) -
Creuzet Nicole,
Berenger JeanFrançois,
Frixon Chantal
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00145.x
Subject(s) - cellobiose , cellulase , cellulose , hydrolysis , thermophile , chemistry , clostridium , biochemistry , phosphoric acid , bacteria , filter paper , enzyme , organic chemistry , biology , chromatography , genetics
A cellobiohydrolase component was isolated from an anaerobic thermophilic cellulolytic bacterium, Clostridium stercorarium . When acting alone, the enzyme showed minimal activity towards ordered substrates such as cellulose and filter paper but it has been shown to attack phosphoric‐acid swollen cellulose giving cellobiose as principal product. When recombined with endoglucanase it did allow an extensive hydrolysis demonstrating a marked synergism in the action of those two components; the addition of β‐glucosidase resulted in a further increase in activity.