Open AccessDo cells treated with human interferon survive virus infection?
Author(s) -
Muñoz Alberto,
Carrasco Luis
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00139.x
Subject(s) - virology , vesicular stomatitis virus , virus , interferon , biology , semliki forest virus , vaccinia , herpes simplex virus , newcastle disease , vesicular stomatitis , hela , microbiology and biotechnology , vesicular stomatitis indiana virus , cell culture , rna , biochemistry , genetics , gene , recombinant dna
HeLa cells pretreated with human lympho‐blastoid interferon (Hu IFN‐α (Ly)), at concentrations up to 100 IU / ml and infected with moderate multiplicities of encephalomyocarditis (EMC) virus (10 PFU / cell) died 1 or 2 days after infection. However, if cells were repeatedly treated with high doses of IFN (800 IU / ml) they survived infection by EMC virus for at least a month. Cells survived Semliki Forest virus (SFV) infection when even lower IFN concentrations were used. By contrast infection of IFN‐treated HeLa cells with other RNA‐containing viruses, such as poliovirus, vesicular stomatitis virus (VSV), Newcastle disease virus (NDV) and reovirus type 3 resulted in cell death. Similarly, infection with a number of DNA‐containing viruses such as adenovirus type 5, Herpes simplex virus types 1 and 2 (HSV‐1 and HSV‐2) and vaccinia virus killed cells. The results are discussed in the light of different models for the molecular mechanism of action of interferon.