Open AccessAffinity of phosphatidylglycerophosphate synthetase for the liponucleotides in Bacillus subtilis
Author(s) -
Gaillard JeanLuc,
Lubochinsky Bernard,
Rigomier Daniel
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00136.x
Subject(s) - phosphatidylglycerol , diglyceride , biochemistry , bacillus subtilis , phospholipid , chemistry , substrate (aquarium) , biosynthesis , enzyme , membrane , biology , bacteria , phosphatidylcholine , ecology , genetics
In Bacillus subtilis , the synthesis of phosphatidylglycerol, the more reactive phospholipid, was investigated in vitro. The phosphatidylglycerophosphate synthetase was exclusively localized in the membrane fraction. Three phospholipids (phosphatidylglycerophosphate, phosphatidylglycerol and diphosphatidylglycerol) were synthesized by this fraction. Exogenous CDP‐diglyceride and dCDP‐diglyceride were substrates with the same K m (0.11 mM). In coupled system with CDP‐diglyceride synthetase, endogenous dCDP‐diglyceride was a less effective substrate than CDP‐diglyceride.