Open AccessEnrichment technique for the selection of catabolite repression‐resistant mutants of Trichoderma as producers of cellulase
Author(s) -
Labudová I.,
Farkaš V.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00119.x
Subject(s) - catabolite repression , trichoderma reesei , cellulase , cellobiose , fed batch culture , repressor , conidium , microbiology and biotechnology , biochemistry , mutant , chemistry , biology , cellulose , botany , fermentation , gene , transcription factor
Abstract The enrichment technique for the preparation of catabolite repression‐resistant producers of cellulase from Trichoderma reesei is based on the submerged cultivation of mutagenized conidia on 2% (w/v) cellobiose or carboxymethyl‐cellulose and in the presence of 0.5% (w/v) 2‐deoxyglucose as the catabolite repressor. Conidia that are resistant towards the catabolite repressor can produce enzymes necessary for hydrolysis of used substrates and grow under the given conditions. They can be separated from the ungerminated conidia by filtration and used for the production of new conidia which are already enriched with catabolite repression‐resistant mutants.