Open AccessCharacterization of a high molecular mass resistance plasmid isolated from Klebsiella pneumoniae and coding for lactose degradation
Author(s) -
Lucain Christine,
Piffaretti JeanClaude
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00102.x
Subject(s) - plasmid , klebsiella pneumoniae , agarose gel electrophoresis , escherichia coli , lactose , microbiology and biotechnology , plasmid preparation , molecular mass , biology , restriction enzyme , gel electrophoresis , enterobacteriaceae , salmonella , dna , bacteria , gene , genetics , biochemistry , enzyme , pbr322
Originated from Klebsiella pneumoniae , pCL300 is a self‐transmissible plasmid, coding for multiple antibiotic resistances and for lactose degradation. This fi + plasmid belongs to the incompatibility group F I and is stably maintained in various lactose‐negative Escherichia coli or in Salmonella typhi . The molecular mass determined by agarose gel electrophoresis after endonuclease digestions and confirmed by the electron microscope was 243 ± 15 kb, an unusually high value for a plasmid belonging to the incF I group.