Open AccessThe membrane potential is the driving force for siderophore iron transport in fungi
Author(s) -
Huschka HansGeorg,
Müller Gertraud,
Winkelmann Günther
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00101.x
Subject(s) - siderophore , depolarization , membrane potential , membrane , biophysics , biochemistry , membrane transport , chemiosmosis , biology , electrochemical gradient , chemistry , atp synthase , enzyme , gene
Respiratory inhibitors and uncouplers severely impair [ 55 Fe]ferricrocin uptake by Neurospora crassa . parallel measurements of ATP decay and ferricrocin uptake, however, disprove the idea that direct input of metabolic energy in the form of ATP is required for transmembrane movement of siderophores. The role of the membrane potential for siderophore uptake was demonstrated using iron‐deficient cells, which were derepressed in the glucose‐II uptake system. Addition of high amounts of glucose (1 mM) to glu‐II‐derepressed cells leads to a membrane depolarization of about 120 mV, followed by a significant inhibition of ferricrocin uptake, which recovered after some minutes. Full transport inhibition occurred after membrane depolarization in the presence of plasma membrane ATP‐ase inhibitors (DCCD or DES), indicating that the membrane potential is essential for siderophore transport in fungi.