Open AccessTrifluoperazine inhibits the infectivity of Chlamydia trachomatis for McCoy cells
Author(s) -
Kihlström Erik,
Söderlund Gustaf
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00100.x
Subject(s) - chlamydia trachomatis , infectivity , microbiology and biotechnology , virology , chlamydia , chlamydiaceae , biology , immunology , virus
Trifluoperazine (TFP), an inhibitor of the Ca 2+ ‐binding protein calmodulin, was used to study the infectivity of Chlamydia trachomatis for McCoy cells. TFP inhibited the number of chlamydial inclusions and the chlamydia‐dependent amino acid incorporation when added within 9 h after inoculation with chlamydiae. However, TFP did not affect the attachment of chlamydiae to the cells or the protease‐removable fraction of cell‐bound chlamydiae. These results suggest that an early step in the intracellular development of chlamydiae, partly coinciding with the elementary body‐reticulate body conversion, is sensitive to TFP and that clathrin coats are not crucial in the ingestion of chlamydiae by McCoy cells.