Open Accessl ‐Methionine‐ dl ‐sulfoximine resistant Het + Nif + and Het − Nif − strains of Nostoc muscorum assimilating methylamine as ammonium nitrogen source
Author(s) -
Singh H.N.,
Bagchi S.N.,
Singh R.K.
Publication year - 1983
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1983.tb00084.x
Subject(s) - glutamine synthetase , methylamine , ammonium , cyanobacteria , biology , glutamine , methionine , microbiology and biotechnology , enzyme , mutant , nostoc , biochemistry , chemistry , bacteria , amino acid , gene , genetics , organic chemistry
The Het + Nif + and Het − Nif − strains of Nostoc muscorum sensitive to growth inhibition by methylamine (MA), overcame the MA inhibition as a result of their mutation to l ‐methionine‐ dl ‐sulfoximide (MSX)‐resistant phenotype, which enabled them to assimilate MA like an ammonium nitrogen source. The MSX‐resistant Het + Nif + strain synthesized the inhibitor‐resistant transferase‐defective glutamine synthetase (GS), which unlike parental GS underwent MA‐dependent in vivo activation. These results suggest the involvement of GS enzyme in control of MA assimilation in cyanobacteria.