Open AccessIn vitro propagation of C andidatus R ickettsia andeanae isolated from A mblyomma maculatum
Author(s) -
LuceFedrow Alison,
Wright Chelsea,
Gaff Holly D.,
Sonenshine Daniel E.,
Hynes Wayne L.,
Richards Allen L.
Publication year - 2012
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00905.x
Subject(s) - candidatus , biology , rickettsia , outbreak , spotted fever , amblyomma , virology , tick borne disease , tick , zoology , microbiology and biotechnology , ixodidae , 16s ribosomal rna , bacteria , genetics , virus
Candidatus R ickettsia andeanae was identified during an investigation of a febrile outbreak in northwestern P eru (2002). DNA sequencing from two ticks ( A mblyomma maculatum , I xodes boliviensis ) collected during the investigation revealed a novel R ickettsia agent with similarity to the spotted fever group rickettsiae. Since then, C andidatus R . andeanae has been detected in A . maculatum ticks collected in the southeastern and southcentral U nited S tates, A rgentina, and P eru. To date, C andidatus R . andeanae has not been successfully cultivated in the laboratory. We present evidence for the continuous cultivation in three cell lines of C andidatus R. andeanae isolated from an A . maculatum tick ( P ortsmouth, V irginia).