Open AccessDevelopment and evaluation of new primers for PCR ‐based identification of type II fimA of P orphyromonas gingivalis
Author(s) -
Moon JiHoi,
Shin SeungIl,
Chung JongHyuk,
Lee SeungWoo,
Amano Atsuo,
Lee JinYong
Publication year - 2012
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00889.x
Subject(s) - porphyromonas gingivalis , biology , primer (cosmetics) , genotype , microbiology and biotechnology , polymerase chain reaction , false positive paradox , periodontitis , gene , bacteria , genetics , medicine , chemistry , organic chemistry , machine learning , computer science
For more accurate PCR ‐based identification of P orphyromonas gingivalis harboring genotype II fimA , the most prevalent type in periodontitis patients, a new primer set was developed and evaluated. The previous type II primers hybridized to the DNA of P . gingivalis strains harboring type I b as well as type II fimA , while the new primers specifically amplified only the DNA fragment of type II fimA . In the investigation using mixed bacterial culture and 155 clinical samples from peri‐implantitis patients, the new primers increased the accuracy of PCR ‐based detection of type II fimA by excluding false‐negatives as well as false‐positives.