Open AccessCharacterization of class I integrons among S almonella enterica serovar E nteritidis isolated from humans and poultry
Author(s) -
Firoozeh Farzaneh,
ZahraeiSalehi Taghi,
Shahcheraghi Fereshteh,
Karimi Vahid,
Aslani Mohammad M.
Publication year - 2012
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00883.x
Subject(s) - biology , salmonella enteritidis , salmonella enterica , microbiology and biotechnology , serotype , salmonella , ciprofloxacin , multiple drug resistance , minimum inhibitory concentration , antibiotic resistance , integron , florfenicol , gene cassette , virology , drug resistance , antibiotics , genetics , bacteria
A total of 84 S almonella enterica serovar E nteritidis ( S . E nteritidis) isolates, 42 of human and 42 of poultry origin, were characterized for antimicrobial resistance patterns and class I integrons. Among them, 58 (69%) S . E nteritidis were multidrug‐resistant ( MDR ) and showed resistance to two or more antibiotic classes. By PCR assays and DNA sequencing, 50 (59.5%) S . E nteritidis isolates were found to carry class I integrons. Amplification of internal variable regions of class I integrons revealed five different arrays (0.75 kb only, 1 kb only, 1.3 kb only, both 1 and 1.2 kb, and both 1 and 1.3 kb). The integrons were further sequenced and the dfrA25 (0.75 kb), aadA1 (1 kb), aadA2 (1 kb), bla PSE1 (1.2 kb) aadA6‐orfD (1.3 kb) gene cassette arrays were identified. Ciprofloxacin minimum inhibitory concentration ( MIC ) values for the three isolates that showed resistance or reduced susceptibility via the disc diffusion method were 0.5–4 μg mL −1 , although only three isolates exhibited resistance to cefteriaxone ( MIC : 128–256 μg mL −1 ) and four isolates were resistant to florfenicol ( MIC : 32–128 μg mL −1 ). In conclusion, the high rates of multidrug‐resistance and class I integrons found among S . E nteritidis isolates in humans and poultry in T ehran suggest that efforts are needed to confine the prevalence of MDR S almonella isolates.