Open AccessRegulation of the IL‐10/IL‐12 axis in human dendritic cells with probiotic bacteria
Author(s) -
Gad Monika,
Ravn Peter,
Søborg Ditte A.,
LundJensen Karina,
Ouwehand Arthur C.,
Jensen Simon S.
Publication year - 2011
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00835.x
Subject(s) - probiotic , proinflammatory cytokine , microbiology and biotechnology , biology , immune system , dendritic cell , secretion , cytokine , interleukin 10 , lactobacillus acidophilus , immunology , bacteria , inflammation , biochemistry , genetics
In this study, we have used monocyte‐derived dendritic cells (DCs) to design a screening model for the selection of microorganisms with the ability to suppress DC‐secreted IL‐12p70, a critical cytokine for the induction of T‐helper cell type 1 immune responses under inflammatory conditions. By the treatment of DCs with cocktails containing TLR agonists and proinflammatory cytokines, the cells increased the secretion of the Th1‐promoting cytokine IL‐12p70. Clinically used probiotics were tested for their IL‐10‐ and IL‐12p70‐stimulating properties in immature DCs, and showed a dose‐dependent change in the IL‐10/IL‐12p70 balance. Lactobacillus acidophilus NCFM ™ and the probiotic mixture VSL#3 showed a strong induction of IL‐12p70, whereas Lactobacillus salivarius Ls‐33 and Bifidobacterium infantis 35624 preferentially induced IL‐10. Escherichia coli Nissle 1917 induced both IL‐10 and IL‐12p70, whereas the probiotic yeast Saccharomyces boulardii induced low levels of cytokines. When combining these microorganisms with the Th1‐promoting cocktails, E. coli Nissle 1917 and B. infantis 35624 were potent suppressors of IL‐12p70 secretion in an IL‐10‐independent manner, indicating a suppressive effect on Th1‐inducing antigen‐presenting cells. The present model, using cocktail‐stimulated DCs with potent IL‐12p70‐stimulating capacity, may be used as an efficient tool to assess the anti‐inflammatory properties of microorganisms for potential clinical use.