Open AccessDectin‐1 mediates in vitro phagocytosis of Candida albicans yeast cells by retinal microglia
Author(s) -
Maneu Victoria,
Yáñez Alberto,
Murciano Celia,
Molina Andrés,
Gil María Luisa,
Gozalbo Daniel
Publication year - 2011
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00829.x
Subject(s) - phagocytosis , microglia , candida albicans , biology , tlr2 , microbiology and biotechnology , corpus albicans , mannose receptor , integrin alpha m , retinal , in vitro , yeast , receptor , macrophage , immunology , innate immune system , biochemistry , inflammation
We have investigated the expression of TLR2 and Dectin‐1 in retinal microglia and their involvement in Candida albicans phagocytosis using a cytometric approach. The expression of both receptors has been demonstrated in CD11b + retinal cells. Phagocytosis of pHrodo‐labelled C. albicans yeasts by microglial CD11b + cells of C57BL/6 mice was inhibited both by the Dectin‐1 antagonist laminarin and anti‐Dectin‐1 antibodies, whereas phagocytosis of yeasts by retinal microglia of TLR2 KO mice was unaffected. These data indicate that phagocytosis of C. albicans yeasts by retinal microglia is mediated by Dectin‐1, whereas TLR2 does not play a significant role in this process.