Open AccessQuantitative analysis of biofilm formation of methicillin‐resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device‐related infections
Author(s) -
Kawamura Hideki,
Nishi Junichiro,
Imuta Naoko,
Tokuda Koichi,
Miyanohara Hiroaki,
Hashiguchi Teruto,
Zenmyo Michihisa,
Yamamoto Takuya,
Ijiri Kosei,
Kawano Yoshifumi,
Komiya Setsuro
Publication year - 2011
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2011.00821.x
Subject(s) - biofilm , microbiology and biotechnology , staphylococcus aureus , genotyping , typing , colonization , micrococcaceae , methicillin resistant staphylococcus aureus , asymptomatic carrier , staphylococcal infections , biology , asymptomatic , medicine , genotype , bacteria , antibacterial agent , antibiotics , biochemistry , genetics , gene
Biofilms play a pivotal role in medical device‐related infections. However, epidemiological analysis of biofilm formation and genotyping among clinical methicillin‐resistant Staphylococcus aureus (MRSA) isolates from patients with orthopaedic infections has rarely been reported. A total of 168 MRSA strains were examined: 23 strains from patients with device‐related infection (the device group); 55 from patients with device‐non‐related infection (the nondevice group); and 90 from asymptomatic nasal carriers (the colonization group). Pulsed‐field gel electrophoresis analysis and five genotyping methods including agr typing were performed. Biofilm formation was quantified using a microtitre plate assay. The device group had a significantly higher incidence of agr ‐2 than the colonization group (78.3% vs. 34.4%, P =0.001). The biofilm index of the agr ‐2 (0.523 ± 0.572) strains was significantly higher than those of agr ‐1 (0.260 ± 0.418, P <0.0001) and agr ‐3 (0.379 ± 0.557, P =0.045). The prevalence of strong biofilm formers in the device group (43.5%) was significantly higher than that in the nondevice group (12.7%, P =0.003) and the colonization group (20.0%, P =0.020). agr ‐2 MRSA strains may be more likely to cause orthopaedic device infection because of their strong biofilm formation ability.