Open AccessCX3CL1 expression induced by Candida albicans in oral fibroblasts
Author(s) -
Ohta Kouji,
Nishi Hiromi,
Fukui Akiko,
Shigeishi Hideo,
Takechi Masaaki,
Kamata Nobuyuki
Publication year - 2010
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2010.00734.x
Subject(s) - cx3cl1 , candida albicans , chemokine , biology , corpus albicans , cxcl10 , microbiology and biotechnology , immune system , immunology , chemokine receptor
Oral fibroblasts as well as keratinocytes are thought to influence host inflammatory responses against Candida albicans . However, little is known about chemokine expressions in oral fibroblasts against C. albicans infection. We therefore examined whether C. albicans induced several chemokines including fractalkine/CX3CL1 (CX3CL1), a unique chemokine that has properties of both chemoattractants and adhesion molecules, in fibroblasts and keratinocytes. The addition of C. albicans live cells to human immortalized oral keratinocytes (RT7) resulted in increases in the mRNA levels of multiple chemokines, but not of CX3CL1. In contrast, live and heat‐killed C. albicans caused an increase in CX3CL1 mRNA and protein expression in human immortalized oral fibroblasts (GT1). CX3CL1 mRNA expression in GT1 cells was also enhanced by stimulation with a nonalbicans species of Candida . Further, the CX3CL1 chemokine domain showed antifungal activity against C. albicans . CX3CL1 secreted by oral fibroblasts appears to play an important role in the oral immune response to C. albicans infection.