Open AccessDetection of the CS20 colonization factor antigen in diffuse‐adhering Escherichia coli strains
Author(s) -
Ochoa Theresa J.,
Rivera Fulton P.,
Bernal Maria,
Meza Rina,
Ecker Lucie,
Gil Ana I.,
Cepeda David,
Mosquito Susan,
Mercado Erik,
Maves Ryan C.,
Hall Eric R.,
Svennerholm AnnMari,
McVeigh Annette,
Savarino Stephen,
Lanata Claudio F.
Publication year - 2010
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2010.00730.x
Subject(s) - microbiology and biotechnology , enterotoxigenic escherichia coli , biology , escherichia coli , antigen , toxin , diarrhea , colonization , enterobacteriaceae , enterotoxin , monoclonal antibody , antibody , virology , immunology , gene , medicine , biochemistry
We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin‐producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot‐blot assay using 21 CF‐specific monoclonal antibodies. Out of 95 non‐ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20‐positive strains as ETEC‐negative by repeat PCR and as toxin‐negative by ganglioside‐GM1‐enzyme‐linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non‐ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli , which may then play a role in pathogenesis and the immune response of the host.