Open AccessIncreasing effect of a high dose of PG‐1 peptide on the infectivity of Chlamydophila abortus
Author(s) -
Donati Manuela,
Di Francesco Antonietta,
Gennaro Renato,
Benincasa Monica,
Di Paolo Maria,
Shurdhi Alisa,
Ostanello Fabio,
Baldelli Raffaella,
Cevenini Roberto
Publication year - 2010
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2010.00679.x
Subject(s) - infectivity , biology , microbiology and biotechnology , chlamydia trachomatis , chlamydiaceae , chlamydiales , chlamydia , serotype , protozoa , antimicrobial , virology , bacteria , in vitro , chlamydiae , peptide , virus , immunology , biochemistry , genetics
Cathelicidins are antimicrobial peptides, stored by mammalian leukocytes, showing an antimicrobial activity against bacteria, fungi, protozoa and enveloped viruses. In accordance with other authors, we reported in a previous study that the protegrin‐1 (PG‐1), at 80 μg mL −1 , inhibited the in vitro growth of Chlamydia trachomatis serovars D, H and L2; however, we observed an increased infectivity of some animal chlamydial species after their treatment with the same PG‐1 concentration. In this study, the treatment of LLC‐MK2 cells with PG‐1 before chlamydial infection resulted in an increased infectivity of Chlamydophila abortus probably due to their easier entry into the host cells, whereas no increase in S26/3 infectivity was detected in LLC‐MK2 cells treated with PG‐1 postchlamydial infection.