Aggregatibacter actinomycetemcomitans accelerates atherosclerosis with an increase in atherogenic factors in spontaneously hyperlipidemic mice

Cariogenic and periodontal pathogens are thought to be etiological factors in the development of cardiovascular disease. We assessed the involvement of the periodontal pathogen Aggregatibacter actinomycetemcomitans and cariogenic pathogen Streptococcus mutans in the development of atherosclerosis in apolipoprotein E‐deficient spontaneously hyperlipidemic (Apoe shl ) mice. The mice were treated intravenously with A. actinomycetemcomitans HK1651, S. mutans GS‐5, or phosphate‐buffered saline three times a week for 3 weeks and killed at 15 weeks of age. The areas of the aortic sinus that were covered with atherosclerotic plaque were significantly larger in Apoe shl mice challenged with A. actinomycetemcomitans compared with S. mutans ‐ or vehicle‐challenged mice. Aggregatibacter actinomycetemcomitans challenge increased serum high‐sensitive C‐reactive protein and lipopolysaccharide levels. Bacterial DNA was detected in the blood, heart, and spleen, but not in the liver. Furthermore, serum interleukin‐6 (IL‐6), IL‐8, tumor necrosis factor α, and MCP‐1 levels and Toll‐like receptor (TLR)2, TLR4, ICAM‐1, E‐selectin, P‐selectin, LOX‐1, HSP60, CCL19, CCL21, CCR7, and MCP‐1 expressions in the aorta were significantly increased in mice challenged with A. actinomycetemcomitans . These results suggest that systemic infection with A. actinomycetemcomitans accelerates atherosclerosis in Apoe shl mice by exposing the whole microorganisms or their products, followed by initiating inflammation. Increases in proatherogenic factors may explain the aggravation of atherosclerosis by A. actinomycetemcomitans infection.