Open AccessIncreased tumour necrosis factor‐α production, higher mannose receptor activity and ability to kill Candida by concanavalin‐A‐activated macrophages
Author(s) -
Geraldino Thais Herrero,
De Vito Eliana,
Custódio Luiz Antonio,
ConchonCosta Ivete,
Gaziri Luis Carlos Jabur,
Felipe Ionice,
Loyola Wagner,
Bonifácio Kamila Landucci
Publication year - 2010
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2010.00655.x
Subject(s) - concanavalin a , candida albicans , biology , mannose receptor , microbiology and biotechnology , fluorescein isothiocyanate , tumor necrosis factor alpha , macrophage , spleen , mannose , receptor , peritoneal cavity , corpus albicans , necrosis , immunology , phagocytosis , biochemistry , in vitro , physics , genetics , anatomy , quantum mechanics , fluorescence
In a previous study, our group verified that mice pretreated with concanavalin‐A (Con‐A) produced more tumour necrosis factor (TNF)‐α and presented greater Candida clearance from the peritoneal cavity, liver and spleen, which yielded a higher survival rate than control animals. In this work, the hypothesis that macrophages were of crucial importance in overcoming the infection was tested. Thus, peritoneal macrophages from mice pretreated for 3 days with Con‐A or phosphate‐buffered saline (PBS) were coincubated with CR1, CR15 and 577 isolates of Candida albicans for 0.5, 1 and 2 h. The ability of Con‐activated macrophages to produce TNF‐α, ingest via mannose receptors and kill all the isolates was significantly greater compared with PBS‐treated macrophages, and activated macrophages exhibited a lower incidence of apoptosis, verified by binding to annexin V‐fluorescein isothiocyanate. The transition of yeast cells to filamentous forms during coincubation for 2 h with control macrophages was about 73–80%, whereas in the presence of Con‐A‐activated macrophages, it was 35–40%. Our results suggest that a greater clearance of C. albicans infection through treatment with Con‐A is probably due to the activation of macrophages, which produce more TNF‐α, express more mannose receptors and are better endowed to kill ingested C. albicans .