Open AccessMolecular characterization of class 1 integrons in MDR Pseudomonas aeruginosa isolated from clinical settings in Iran, Tehran
Author(s) -
Shahcheraghi Fereshteh,
Badmasti Farzad,
Feizabadi Mohammad M.
Publication year - 2010
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2009.00636.x
Subject(s) - pseudomonas aeruginosa , gene cassette , biology , microbiology and biotechnology , gene , integron , multiple drug resistance , genetics , drug resistance , antibiotics , antibiotic resistance , bacteria
Seven hundred and fifty nonreplicated clinical isolates of Pseudomonas aeruginosa were collected from five hospitals during March 2007 to May 2009. Forty‐one isolates (5.46%) were characterized as multidrug‐resistant P. aeruginosa (MDRPA). PCR assays were used to detect class 1 integrons. Amplifications of internal variable regions (IVRs) of class 1 integrons revealed three different arrays (0.8, 1.3, and 1.7 kb) with different distributions in clinical isolates. The amplified IVRs were sequenced and three gene cassette arrays including aadB (0.8 kb), aadA6–orfD (1.3 kb), and bla OXA10 –aacA4 (1.7 kb) were identified. In conclusion, we confirmed the high prevalence of class 1 integons with limited diversity of gene cassette arrays in MDRPA clinical isolates found from five hospitals. This is the first report showing gene cassette contents of class 1 integrons in P. aeruginosa isolates in Iran.