Open AccessPseudomonas aeruginosa quorum‐sensing signal molecules interfere with dendritic cell‐induced T‐cell proliferation
Author(s) -
Skindersoe Mette E.,
Zeuthen Louise H.,
Brix Susanne,
Fink Lisbeth N.,
Lazenby James,
Whittall Christine,
Williams Paul,
Diggle Stephen P.,
Froekiaer Hanne,
Cooley Margaret,
Givskov Michael
Publication year - 2009
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00533.x
Subject(s) - quorum sensing , pseudomonas aeruginosa , biology , immune system , microbiology and biotechnology , lipopolysaccharide , cell signaling , proinflammatory cytokine , cytokine , t cell , dendritic cell , virulence , signal transduction , bacteria , immunology , inflammation , biochemistry , genetics , gene
Pseudomonas aeruginosa releases a wide array of toxins and tissue‐degrading enzymes. Production of these malicious virulence factors is controlled by interbacterial communication in a process known as quorum sensing. An increasing body of evidence reveals that the bacterial signal molecule N ‐(3‐oxododecanoyl)‐ l ‐homoserine lactone (OdDHL) exhibits both quorum‐sensing signalling and immune‐modulating properties. Recently, yet another quorum‐sensing signal molecule, the Pseudomonas quinolone signal (PQS), has been shown to affect cytokine release by mitogen‐stimulated human T cells. In the present article we demonstrate that both OdDHL and PQS decrease the production of interleukin‐12 (IL‐12) by Escherichia coli lipopolysaccharide‐stimulated bone marrow‐derived dendritic cells (BM‐DCs) without altering their IL‐10 release. Moreover, BM‐DCs exposed to PQS and OdDHL during antigen stimulation exhibit a decreased ability to induce T‐cell proliferation in vitro . Collectively, this suggests that OdDHL and PQS change the maturation pattern of stimulated DCs away from a proinflammatory T‐helper type I directing response, thereby decreasing the antibacterial activity of the adaptive immune defence. OdDHL and PQS thus seem to possess dual activities in the infection process: as inducers of virulence factors as well as immune‐modulators facilitating the infective properties of this pathogen.