Open AccessA limited role for antibody in protective immunity induced by rCPAF and CpG vaccination against primary genital Chlamydia muridarum challenge
Author(s) -
Murthy Ashlesh K.,
Chaganty Bharat K.R.,
Li Weidang,
Guentzel M. Neal,
Chambers James P.,
Seshu J.,
Zhong Guangming,
Arulanandam Bernard P.
Publication year - 2009
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00517.x
Subject(s) - chlamydia , biology , vaccination , antibody , immunology , immunity , cpg oligodeoxynucleotide , virology , immune system , biochemistry , gene expression , dna methylation , gene
Mice deficient in B cells (μmT mice) were used to evaluate the role of antibody in enhanced chlamydial clearance and reduction of pathology afforded by vaccination with recombinant chlamydial protease‐like activity factor (rCPAF). Enhanced, but comparable, chlamydial clearance was observed in μmT and wild‐type (WT) mice after rCPAF+CpG vaccination. Chlamydia ‐induced pathology was present in mock‐immunized animals, but at significantly greater levels in μmT than WT mice, whereas vaccinated μmT and WT mice exhibited similar reductions in pathology. Thus, antibodies may play a role in protection against chlamydial pathology after primary infection, but were largely dispensable in rCPAF+CpG‐induced chlamydial clearance and reduction in pathology.